Rat MMP-9/MMP9 (His Tag) recombinant protein

Cat.#: 500128

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Product Information

  • Product Name
    Rat MMP-9/MMP9 (His Tag) recombinant protein
  • Documents
  • Description
    Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils, and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
  • Protein name
    Matrix metallopeptidase 9
  • Protein short names
    CLG4B; MMP-9; MANDP2; GELB; RP23-61O3.10; PRO-MMP-9; B/MMP9; MMP9; AW743869
  • Uniprot ID
    D3ZYK8
  • Gene Name
    Mmp9; rCG_32078
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the rat MMP9 (EDL96479.1) (Met 1-Pro 708) was expressed, fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Rat
  • Molecular weight
    The secreted recombinant rat MMP9 comprises 700 amino acids and predicts a molecular mass of 77.8 kDa. The apparent molecular mass of the rat MMP9 is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is >1000 pmoles/min/μg.
  • Validations

    Rat MMP-9 / CLG4B Protein (His Tag) SDS-PAGE

    Rat MMP-9 / CLG4B Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"