Human IL-1R1/CD121a (Fc Tag) recombinant protein

Human IL-1R1/CD121a (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.

Interleukin-1 receptor type 1

CD121B; CD121A; IL1R; RATTUS NORVEGICUS; IL-1R-1; IL-1R-ALPHA; IL-1RT1; IL-1R1; P80; IL-1RT-1; IL1RA; IL1R-1; INTERLEUKIN 1 BETA RECEPTOR TYPE 1 SHORT; CD121 ANTIGEN-LIKE FAMILY MEMBER A; IL-IR; D2S1473

P14778

IL1R1; IL1R; IL1RA; IL1RT1

Human Cells

A DNA sequence encoding the extracellular domain of human IL1R1 (NP_000868.1) (Met 1-Thr 332) was fused with human IgG1 Fc region at the C-terminus.

Human

The recombinant human IL1R1/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 553 amino acids after removal of the signal peptide and has a predicted molecular mass of 63 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human IL1-beta at 20 μg/ml (100 μl/well) can bind human IL1R1 Fc chimera with a linear ranger of 6.4-160 ng/ml.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized human IL1B-his at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC₅₀ of human IL1R1-Fc is 0.198 μg/mL.
4. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1-Fc. The EC₅₀ of human IL1R1-Fc is 0.48 μg/mL.
5. Measured by its ability to bind canine IL1B in a functional ELISA.
6. Measured by its ability to bind mouse IL1B in a functional ELISA.