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Product Name
Human ICAM-1/CD54 recombinant protein
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Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
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Protein name
Intercellular adhesion molecule 1
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Protein short names
ICAM1; MGC6195; BB2; ICAM-1; LY-47; P3.58; CD54; MALA-2; MYD10
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Uniprot ID
P05362
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Gene Name
ICAM1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) with five amino acids (DDDDK) at the C-terminus was expressed and purified.
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Protein Species
Human
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Molecular weight
The recombinant human ICAM1 consists of 459 amino acids and has a predicted molecular mass of 51 kDa. As a result of glycosylation, it migrates with an apparent molecular mass of 70-80 kDa in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to human ICAM1 coated plates (12.5 μg/ml, 100 μl/well), approximately 20-40% will adhere specifically.
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Validations
Human ICAM-1 / CD54 Protein SDS-PAGE
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