Mouse ICOS Ligand/B7-H2 (His Tag) recombinant protein
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.
Protein short names
B7RP-1; ICOSL; B7H; CD275; KIAA0653; ICOS-L; B7-H2; AU044799; LY115L; GI50; ICOSLG; GL50-B; BG071784; LICOS; MKIAA0653; GL50; B7H2; B7RP1; ICOS LIGAND
A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with a polyhistidine tag at the C-terminus.
The recombinant mouse B7-H2 comprises 244 amino acids with a predicted molecular mass of 27.8 kDa. As a result of glycosylation, the apparent molecular mass of rmB7-H2 is approximately 45-55 kDa in SDS-PAGE under reducing conditions.
> 95 % as determined by SDS-PAGE
Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 40-1000 ng/ml.
Mouse ICOS Ligand / B7-H2 / ICOSLG Protein (His Tag) SDS-PAGE
Related Products / Services
- Recombinant Proteins
- cDNA Clones
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