Mouse ICOS Ligand/B7-H2 (His Tag) recombinant protein

Mouse ICOS Ligand/B7-H2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. Present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production and germinal center formation.

Icos ligand

B7RP-1; ICOSL; B7H; CD275; KIAA0653; ICOS-L; B7-H2; AU044799; LY115L; GI50; ICOSLG; GL50-B; BG071784; LICOS; MKIAA0653; GL50; B7H2; B7RP1; ICOS LIGAND

Q9JHJ8

Icosl; mCG_5977

Human Cells

A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse B7-H2 comprises 244 amino acids with a predicted molecular mass of 27.8 kDa. As a result of glycosylation, the apparent molecular mass of rmB7-H2 is approximately 45-55 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 40-1000 ng/ml.