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Product Name
Human TAOK3/JIK (aa 1-411, His & GST Tag) recombinant protein
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Description
Serine/threonine-protein kinase that acts as a regulator of the p38/MAPK14 stress-activated MAPK cascade and of the MAPK8/JNK cascade. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Inhibits basal activity of MAPK8/JNK cascade and diminishes its activation in response epidermal growth factor (EGF). {PubMed:10559204, PubMed:10924369, PubMed:17396146}.
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Protein name
Serine/threonine-protein kinase TAO3
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Protein short names
DPK; JIK; KDS; MAP3K18
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Uniprot ID
Q9H2K8
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Gene Name
TAOK3; DPK; JIK; KDS; MAP3K18
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the amino acids (Met 1-Asp 411) of human TAOK3 (Q9H2K8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human TAOK3 (aa 1-411)/GST chimera consists of 648 amino acids and has a calculated molecular mass of 74.3 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 92 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 10 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.
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Validations
Human TAOK3 / JIK / MAP3K18 Protein (aa 1-411, His & GST Tag) SDS-PAGE
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