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  • Human R-Spondin 1 / RSPO1 (His Tag) recombinant protein

Human R-Spondin 1 / RSPO1 (His Tag) recombinant protein

Cat.#: 504120

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Product Information

  • Product Name
    Human R-Spondin 1 / RSPO1 (His Tag) recombinant protein
  • Documents
  • Description
    RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
  • Protein name
    R-spondin-1
  • Protein short names
    RP23-325M14.2; CRISTIN3; RSPO1; R-SPONDIN; RSPONDIN; FLJ40906; RP11-566C13.1; RSPO; R-SPONDIN1
  • Uniprot ID
    Q2MKA7
  • Gene Name
    RSPO1
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human RSPO1 (NP_001033722.1) (Met 1-Ala 263) was expressed, fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The secreted recombinant human RSPO1 comprises 254 amino acids with a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 98 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear ranger of 32-800 ng/ml.
    2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 with a linear ranger of 6.4-800 ng/ml.
    3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.05-0.5 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
  • Validations

    Human R-Spondin 1 / RSPO1 Protein (His Tag) SDS-PAGE

    Human R-Spondin 1 / RSPO1 Protein (His Tag) SDS-PAGE

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