Human MAPKAPK5 (His & GST Tag) recombinant protein

Cat.#: 500069

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Product Information

  • Product Name
    Human MAPKAPK5 (His & GST Tag) recombinant protein
  • Documents
  • Description
    Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. {PubMed:17254968, PubMed:17728103, PubMed:19166925, PubMed:21329882, PubMed:9628874}.
  • Protein name
    MAP kinase-activated protein kinase 5
  • Protein short names
    PRAK
  • Uniprot ID
    Q8IW41
  • Gene Name
    MAPKAPK5; PRAK
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human MAPKAPK5/GST chimera consists of 708 amino acids and has a calculated molecular mass of 82 KDa. It migrates as an approximately 75KDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 76 % as determined by SDS-PAGE
  • Activity
    Kinase activity untested
  • Validations

    Human MAPKAPK5 Protein (His & GST Tag) SDS-PAGE

    Human MAPKAPK5 Protein (His & GST Tag) SDS-PAGE

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