Human IFNA4 (Fc Tag) recombinant protein

Human IFNA4 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Interferon alpha-4

IFA4; MGC143607; MUIFN-ALPHA 4; MGC142200; RP23-139P14.30; IFNA4

P05014

IFNA4

Human Cells

A DNA sequence encoding the mature form of human IFN-?4a (NP_066546.1) (Cys 23-Asp 189) was expressed with the N-terminal fused Fc region of human IgG1.

Human

The recombinant human Fc/IFN-?4a is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a predicted molecular mass of 46 kDa. As a result of glycosylation, the apparent molecular mass of rh Fc/IFN-?4a monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV).
The EC₅₀ for this effect is 0.2-1 ng/mL.