Human CDC2 & CCNE1 recombinant protein
A DNA sequence encoding the human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterotrimer was purified.
The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
> 90 % as determined by SDS-PAGE
The specific activity was determined to be 150 nmol/min/mg using Histone H1 as substrate.
Human CDC2 & CCNE1 Heterotrimer Protein SDS-PAGE
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