Human CD86/B7-2 (His & Fc Tag) recombinant protein

Human CD86/B7-2 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.; (Microbial infection) Acts as a receptor for adenovirus subgroup B. {PubMed:16920215}.

T-lymphocyte activation antigen CD86

CD28LG2

P42081

CD86; CD28LG2

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-His 239) of human B7-2 (NP_008820.2) pro-protein was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human B7-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 467 amino acids and has a predicted molecular mass of 53.2 kDa. In SDS-PAGE, the apparent molecular mass of rh B7-2/Fc monomer is approximately 80-90 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind human CD28 in a functional ELISA.
2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.
The ED₅₀ for this effect is 1-5μg/mL.