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  • Human CD80/B7-1 (His & Fc Tag) recombinant protein

Human CD80/B7-1 (His & Fc Tag) recombinant protein

Cat.#: 503507

Special Price 342.0 USD

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Product Information

  • Product Name
    Human CD80/B7-1 (His & Fc Tag) recombinant protein
  • Documents
  • Description
    The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • Protein name
    CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), isoform CRA_a
  • Protein short names
    B7; TSA1; B71; CD28LG1; CD28LG; B7.1; B7-1; LAB7; LY-53; MIC17; LY53; BB1; CD80; CD28L
  • Uniprot ID
    P33681
  • Gene Name
    CD80; hCG_1780136
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human B7-1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. By SDS-PAGE, the apparent molecular mass of rh B7-1/Fc is approximately 80-90 kDa due to the glycosylation.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    1. Measured by its ability to bind human CD28 in a functional ELISA.
    2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.
    The ED50 for this effect is 0.2-0.5μg/mL
  • Validations

    Human CD80 / B7-1 Protein (His & Fc Tag) SDS-PAGE

    Human CD80 / B7-1 Protein (His & Fc Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"