Human CD40/TNFRSF5 (His & Fc Tag) recombinant protein

Human CD40/TNFRSF5 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Tumor necrosis factor receptor superfamily member 5

RP23-428M13.1; TRAP; GP39; MGC9013; BP50; HIGM1; CD40; TNFRSF5; IGM; T-BAM; IMD3; AI326936; CDW40; P50

Q6P2H9

CD40; TNFRSF5

Human Cells

A DNA sequence encoding the human CD40 (NP_001241.1) extracellular domain (Met 1-Arg193) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human CD40/Fc is a disulfide-linked homodimer. The reduced monomer consists of 421 amino acids and has a predicted molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rhCD40/Fc monomer migrates with an apparent molecular mass of 55-60 kDa in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD40 at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 7.8-125 ng/ml.