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Product Name
Human CD32a / Fc gamma RIIA / FCGR2A (167 His, His Tag) recombinant protein
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Protein name
Low affinity immunoglobulin gamma Fc region receptor II-a
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Protein short names
RP11-5K23.6; FCGR21
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Uniprot ID
P12318
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Gene Name
FCGR2A; CD32; FCG2; FCGR2A1; IGFR2
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Source/Expression Host
CHO Cells
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Expression Plasmid/cDNA
A DNA sequence encoding (Met 1-Ile 218) of human CD32a (NP_001129691.1) was expressed with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.
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Protein Species
Human
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Molecular weight
The secreted recombinant human CD32a consists of 196 amino acids and predicts a molecular mass of 22 KDa. The apparent molecular mass of the protein is approximately 29-32 KDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized human CD32a-His (R131H) (CHO) (Cat:503412) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.09-0.21 μg/ml. -
Validations
Human CD32a / FCGR2A Protein (167 His, His Tag) SDS-PAGE
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