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  • Human Carboxypeptidase A2/CPA2 (His Tag) recombinant protein

Human Carboxypeptidase A2/CPA2 (His Tag) recombinant protein

Cat.#: 503281

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Product Information

  • Product Name
    Human Carboxypeptidase A2/CPA2 (His Tag) recombinant protein
  • Documents
  • Description
    Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
  • Protein name
    Carboxypeptidase A1
  • Protein short names
    CPA2; CARBOXYPEPTIDASE A2
  • Uniprot ID
    P15085
  • Gene Name
    CPA1; CPA
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the pre pro form of human CPA2 (NP_001859.1) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
  • Protein Species
    Human
  • Molecular weight
    The secreted recombinant human CPA2 existing as the proform consists of 412 amino acids and has a predicted molecular mass of 46 kDa also as estimated by SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-1. The specific activity is >4,000 pmoles/min/μg.
  • Validations

    Human Carboxypeptidase A2 / CPA2 Protein (His Tag) SDS-PAGE

    Human Carboxypeptidase A2 / CPA2 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"