Canine aFGF / FGF1 recombinant protein

Canine aFGF / FGF1 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1-4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

Fibroblast growth factor

FGF

J9NTP4

FGF1

E. coli

A DNA sequence encoding the canine FGF1 (XP_849274.1) (Phe16-Asp155) was expressed with a N-terminal Met.

Canine

The recombinant canine FGF1 comprises 141 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 16 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts.
The ED₅₀ for this effect is typically 0.05-0.3 ng/ml.