Mouse EGFR/HER1/ErbB1 (His Tag) recombinant protein

Mouse EGFR/HER1/ErbB1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Epidermal growth factor receptor

PIG61; MENA; HER1; ERBB-1; 9030024J15RIK; WA5; ERBB1; ERRP; WA2; NISBD2; ERBB; ERRB1; RP23-295E4.1; WA-2; AI552599; EGFR

Q01279

Egfr

Human Cells

A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  human EGF-Fc (Cat:503608), The EC₅₀ of human EGF-Fc (Cat:503608) is 60-90 ng/mL.
3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  mouse EGF-Fc (Cat:503939), The EC₅₀ of mouse EGF-Fc (Cat:503939) is 70-100 ng/mL.