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  • Mouse EGFR/HER1/ErbB1 (His Tag) recombinant protein

Mouse EGFR/HER1/ErbB1 (His Tag) recombinant protein

Cat.#: 501704

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Product Information

  • Product Name
    Mouse EGFR/HER1/ErbB1 (His Tag) recombinant protein
  • Documents
  • Description
    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  • Protein name
    Epidermal growth factor receptor
  • Protein short names
    PIG61; MENA; HER1; ERBB-1; 9030024J15RIK; WA5; ERBB1; ERRP; WA2; NISBD2; ERBB; ERRB1; RP23-295E4.1; WA-2; AI552599; EGFR
  • Uniprot ID
    Q01279
  • Gene Name
    Egfr
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Mouse
  • Molecular weight
    The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA.
    2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  human EGF-Fc (Cat:503608), The EC50 of human EGF-Fc (Cat:503608) is 60-90 ng/mL.
    3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  mouse EGF-Fc (Cat:503939), The EC50 of mouse EGF-Fc (Cat:503939) is 70-100 ng/mL.
  • Validations

    Mouse EGFR / HER1 / ErbB1 Protein (His Tag) SDS-PAGE

    Mouse EGFR / HER1 / ErbB1 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"