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  • Human EGFR/HER1/ErbB1 (His Tag) recombinant protein

Human EGFR/HER1/ErbB1 (His Tag) recombinant protein

Cat.#: 500195

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Product Information

  • Product Name
    Human EGFR/HER1/ErbB1 (His Tag) recombinant protein
  • Documents
  • Description
    Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  • Protein name
    Epidermal growth factor receptor
  • Protein short names
    RP23-295E4.1; WA-2; AI552599; EGFR; ERBB1; ERRP; WA2; NISBD2; ERBB; ERRB1; ERBB-1; 9030024J15RIK; WA5; PIG61; MENA; HER1
  • Uniprot ID
    P00533
  • Gene Name
    EGFR; ERBB; ERBB1; HER1
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Met 1-Lys 642) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human EGFR consists of 626 amino acids and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its binding ability in a functional ELISA.
    Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 3.2-400 ng/ml.
  • Validations

    Human EGFR / HER1 / ErbB1 Protein (His Tag) SDS-PAGE

    Human EGFR / HER1 / ErbB1 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"