DMRTA2 Antibody (C-term)
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Human, Mouse, Zebrafish
DMRT5 antibody; DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5 antibody
This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
WB: 1:1000, IF: 1:10~50
Western blot analysis of lysates from K562 cell line,zebra fish brain,mouse brain,mouse testis tissue lysate,PC-3 cell line (from left to right), using DMRTA2 Antibody (C-term)(Cat. #126486). 126486 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#126486).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
May be involved in sexual development.
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