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Product Name
Human SMAC/Diablo (His Tag) recombinant protein
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Description
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
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Protein name
Diablo homolog, mitochondrial
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Protein short names
0610041G12RIK; DIABLO-S; DIABLO; SMAC; 1700006L01RIK; AU040403; HCG_1782202; FLJ25049; FLJ10537; DFNA64
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Uniprot ID
Q9NR28
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Gene Name
DIABLO; SMAC
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human Diablo/SMAC consists of 191 amino acids and migrates as polypeptide of 22 kDa as estimated by SDS-PAGE under reduced and non-reduced conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human SMAC-His (Cat:500785）at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (Cat:502786) with a linear range of 0.125-1.0 μg/ml. -
Validations
Human SMAC / Diablo Protein (His Tag) SDS-PAGE
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