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  • CDK1 Human  cDNA/ORF Clones

CDK1 Human cDNA/ORF Clones

Cat.#: 714743-1

  • Accession#:
  • Vector:
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Product Information

  • Gene name
    cyclin dependent kinase 1
  • Organism
    Human
  • Entrez Gene ID
  • Gene Symbol
    CDK1
  • Gene Type
    protein coding
  • Also Known as
    CDC2; CDC28A; P34CDC2
  • Encoded Protein Alias
    cyclin-dependent kinase 1; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase
  • Product Name
    CDK1(NM_001170407) Human cDNA/ORF Clones
  • Accession NO.
  • Transcript Name
    Homo sapiens cyclin dependent kinase 1 (CDK1), transcript variant 4, mRNA.
  • Synonyms
  • ORF length
    330
  • Annotation
    This variant (4) differs in the 5' UTR, 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (4) has a distinct C-terminus and is shorter than isoform 1. Both variants 4 and 5 encode the same isoform (4).
  • siRNA

    GAACUUCGUCAUCCAAAUAtt

    GGUUAUAUCUCAUCUUUGAtt

  • DataSheet:
  • Product Name
    CDK1(NM_033379) Human cDNA/ORF Clones
  • Accession NO.
  • Transcript Name
    Homo sapiens cyclin dependent kinase 1 (CDK1), transcript variant 2, mRNA.
  • Synonyms
  • ORF length
    723
  • Annotation
    This variant (2), also known as CDC2 delta T, lacks an alternate in-frame exon in the middle portion of the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
  • siRNA

    GAACUUCGUCAUCCAAAUAtt

    GGUUAUAUCUCAUCUUUGAtt

    GAAUCUUUACAGGACUAUAtt

  • DataSheet:
  • Vector Name
    pEXP-Entry
  • primers
    T7: TAATACGACTCACTATAGG
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    4919
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Kanamycin
  • Selection Marker
    Neomycin
  • Protein Tag
    Myc,FLAG
  • Description
    ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
    Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
  • Vector Name
    pOTENT-1
  • primers
    pOT-F: TAATAGTAATCAATTACGGG
    pOT-R: CCTCTACAAATGTGGTATGGC
  • Vector Length
    7510
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Kanamycin
  • Selection Marker
    Neomycin
  • Protein Tag
    FLAG, 6 His
  • Description
    ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
    Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
  • Vector Name
    pUC18c
  • primers
    M13 fwd: GTAAAACGACGGCCAGT
    M13 rev: CAGGAAACAGCTATGAC
  • Vector Length
    2693
  • Vector Type
    Cloning Vector
  • Promoter
    lac
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    -
  • Protein Tag
    None
  • Description
    pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
  • Vector Name
    pcDNA3.1-3xFlag
  • primers
    T7: TAATACGACTCACTATAGGG
    BGH: TAGAAGGCACAGTCGAGG
  • Vector Length
    5508
  • Vector Type
    Mammalian Expression Vector
  • Promoter
    CMV
  • Antibiotic Resistance
    Ampicillin
  • Selection Marker
    Neomycin
  • Protein Tag
    Flag
  • Description
    Mammalian vector for expressing C-terminal 3xFlag tagged proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"