-
Gene name
CD86 molecule
-
Organism
Human
- Entrez Gene ID
-
Gene Symbol
CD86
-
Gene Type
protein coding
-
Also Known as
B70; B7-2; B7.2; LAB72; CD28LG2
-
Encoded Protein Alias
T-lymphocyte activation antigen CD86; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; FUN-1
Accession NO.: NM_176892
-
Product Name
CD86(NM_176892) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens CD86 molecule (CD86), transcript variant 3, mRNA.
-
Synonyms
-
ORF length
828
-
Annotation
This variant (3) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. It also lacks an in-frame exon compared to variant 1. The resulting isoform (3, also known as CD86deltaTM) has a shorter N-terminus and lacks an internal segment compared to isoform 1. This isoform is the soluble form of this protein.
-
siRNA
GCACAAGUUUUGAUUCGGAtt
GAAUUCAACUAUCGAGUAUtt
CGAGCAAUAUGACCAUCUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_006889
-
Product Name
CD86(NM_006889) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens CD86 molecule (CD86), transcript variant 2, mRNA.
-
Synonyms
-
ORF length
972
-
Annotation
This variant (2) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. Isoform 2 has a shorter N-terminus than isoform 1.
-
siRNA
GCACAAGUUUUGAUUCGGAtt
GAAUUCAACUAUCGAGUAUtt
CGAGCAAUAUGACCAUCUUtt
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: NM_175862
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Product Name
CD86(NM_175862) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens CD86 molecule (CD86), transcript variant 1, mRNA.
-
Synonyms
-
ORF length
990
-
Annotation
This variant (1) encodes the longest isoform (1) of this protein.
-
siRNA
GCACAAGUUUUGAUUCGGAtt
GAAUUCAACUAUCGAGUAUtt
CGAGCAAUAUGACCAUCUUtt
- DataSheet:
- DataSheet:
- FAQs:
Accession NO.: NM_001206925
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Product Name
CD86(NM_001206925) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens CD86 molecule (CD86), transcript variant 5, mRNA.
-
Synonyms
-
ORF length
744
-
Annotation
This variant (5) lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (5) is made from a downstream translation start site compared to isoform 1.
-
siRNA
GCACAAGUUUUGAUUCGGAtt
GAAUUCAACUAUCGAGUAUtt
CGAGCAAUAUGACCAUCUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_001206924
-
Product Name
CD86(NM_001206924) Human cDNA/ORF Clone
- Accession NO.
-
Transcript Name
Homo sapiens CD86 molecule (CD86), transcript variant 4, mRNA.
-
Synonyms
-
ORF length
654
-
Annotation
This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.
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siRNA
GAAUUCAACUAUCGAGUAUtt
CGAGCAAUAUGACCAUCUUtt
- DataSheet:
- FAQs:
-
Vector Name
pCMV-SPORT6
-
primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4396
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
Vector for cloning and transient mammalian cell expression of cDNAs.
-
Vector Name
pEXP-C-EGFP
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6703
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
GFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6013
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-HA
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6016
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
HA
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-C-His
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6019
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
His
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-C-mOrange2
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6662
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
OFPSpark
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
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Vector Name
pEXP-SP-N-FLAG
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6058
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
FLAG
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-SP-N-HA
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6061
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
HA
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-SP-N-His
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6064
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
His
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-SP-N-Myc
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6064
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Hygromycin
-
Protein Tag
Myc
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pEXP-untagged
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
6223
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Hygromycin
-
Protein Tag
None
-
Description
All of the pEXP vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
• Hygromycin resistance gene for selection of mammalian cell lines.
• A Kozak consensus sequence to enhance mammalian expression.
-
Vector Name
pGEM-T
-
primers
T7: TAATACGACTCACTATAGGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
3000
-
Vector Type
Cloning Vector
-
Promoter
lac
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
-
-
Protein Tag
None
-
Description
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
-
Vector Name
pOTENT-1
-
primers
pOT-F: TAATAGTAATCAATTACGGG
pOT-R: CCTCTACAAATGTGGTATGGC -
Vector Length
7510
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Kanamycin
-
Selection Marker
Neomycin
-
Protein Tag
FLAG, 6 His
-
Description
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal FLAG-6 His tags.
Such clones are the best for detection and purification of the transgene using anti-FLAG or anti-His antibodies.
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Vector Name
pcDNA3.1-3xFlag
-
primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5494
-
Vector Type
Mammalian Expression Vector
-
Promoter
CMV
-
Antibiotic Resistance
Ampicillin
-
Selection Marker
Neomycin
-
Protein Tag
Flag
-
Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"