Rat CD86/B7-2 (His Tag) recombinant protein

Rat CD86/B7-2 (His Tag) recombinant protein

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CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Cd86 antigen, isoform CRA_c

O35531

Cd86; rCG_52586

Human Cells

A DNA sequence encoding the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat CD86 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. The apparent molecular mass of the rat CD86 is approximately 40-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-160 ng/ml.