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  • Human Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein

Human Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein

Cat.#: 501946

Special Price 238.2 USD

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Product Information

  • Product Name
    Human Carbonic Anhydrase IX/CA9 (His Tag) recombinant protein
  • Documents
  • Description
    Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
  • Protein name
    Carbonic anhydrase 9
  • Protein short names
    CA9; CARBONIC ANHYDRASE IX; CAIX; MN/CA9; CAR9; CARBONIC ANHYDRASE 9; MN; RP23-195K8.9
  • Uniprot ID
    Q16790
  • Gene Name
    CA9; G250; MN
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a polyhistidine tag at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human CA9 consists of 388 amino acids and predicts a molecular mass of 42.5 kDa. As a result of glycosylation, rhCA9 migrates with apparent molecular mass of 48 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its esterase activity. The specific activity is >30 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
  • Validations

    Human Carbonic Anhydrase IX / CA9 Protein (His Tag) SDS-PAGE

    Human Carbonic Anhydrase IX / CA9 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"