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Product Name
Human Carbonic Anhydrase XII (His Tag) recombinant protein
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Description
Reversible hydration of carbon dioxide.
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Protein name
Carbonic anhydrase 12
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Protein short names
AI314958; FLJ20151; CARBONIC ANHYDRASE 12; CAR12; HST18816; 2310047E01RIK; CAXII; CARBONIC ANHYDRASE XII; CA12
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Uniprot ID
O43570
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Gene Name
CA12
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
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Validations
Human Carbonic Anhydrase XII / CA12 Protein (His Tag) SDS-PAGE
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