Cynomolgus CD80/B7-1 (Fc Tag) recombinant protein

Cynomolgus CD80/B7-1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

B7 protein

O77684

B7

Human Cells

A DNA sequence encoding the cynomolgus CD80 (AAC31555.1) (Met1-Asn242) was expressed with the Fc region of human IgG1 at the C-terminus.

Cynomolgus

The recombinant cynomolgus CD80 is a disulfide-linked homodimer. The reduced monomer comprises 449 amino acids and has a calculated molecular mass of 50.9 KDa.The apparent molecular mass of the protein is approximately 65 and 44 KDa respectively in SDS-PAGE.

(89.4+9.8) % as determined by SDS-PAGE

Immobilized Cynomolgus CTLA4-His (Cat:503512) at 10 μg/ml (100 μl/well) can bind Cynomolgus CD80-Fc, The EC₅₀ of CynomolgusCD80-Fc is 0.02-0.04 μg/ml.