Cynomolgus CD80/B7-1 (Fc Tag) recombinant protein
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
A DNA sequence encoding the cynomolgus CD80 (AAC31555.1) (Met1-Asn242) was expressed with the Fc region of human IgG1 at the C-terminus.
The recombinant cynomolgus CD80 is a disulfide-linked homodimer. The reduced monomer comprises 449 amino acids and has a calculated molecular mass of 50.9 KDa.The apparent molecular mass of the protein is approximately 65 and 44 KDa respectively in SDS-PAGE.
(89.4+9.8) % as determined by SDS-PAGE
Immobilized Cynomolgus CTLA4-His (Cat:503512) at 10 μg/ml (100 μl/well) can bind Cynomolgus CD80-Fc, The EC50 of CynomolgusCD80-Fc is 0.02-0.04 μg/ml.
Cynomolgus CD80 / B7-1 Protein (Fc Tag) SDS-PAGE
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