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Gene name
argonaute 3, RISC catalytic component
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Organism
Human
- Entrez Gene ID
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Gene Symbol
AGO3
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Gene Type
protein coding
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Also Known as
EIF2C3
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Encoded Protein Alias
protein argonaute-3; argonaute 3; eukaryotic translation initiation factor 2C, 3; hAgo3
Accession NO.: XM_011540879
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Product Name
AGO3(XM_011540879) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
PREDICTED: Homo sapiens argonaute 3, RISC catalytic component (AGO3), transcript variant X3, mRNA.
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Synonyms
-
ORF length
2538
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siRNA
GAAGAGACAUCACACUCGAtt
CAGUCGUCCUUCACACUAUtt
CCAUAUGAGUUCGAUUUUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_177422
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Product Name
AGO3(NM_177422) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens argonaute 3, RISC catalytic component (AGO3), transcript variant 2, mRNA.
- Synonyms
-
ORF length
1881
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Annotation
This variant (2) has multiple differences in the coding region, which causes translation initiation at a downstream start codon, compared to variant 1. The resulting protein (isoform b) has a shorter N-terminus than isoform a.
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siRNA
GAAGAGACAUCACACUCGAtt
CAGUCGUCCUUCACACUAUtt
CCAUAUGAGUUCGAUUUUUtt
- DataSheet:
- FAQs:
Accession NO.: XM_017000528
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Product Name
AGO3(XM_017000528) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
PREDICTED: Homo sapiens argonaute 3, RISC catalytic component (AGO3), transcript variant X10, mRNA.
- Synonyms
-
ORF length
1758
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siRNA
GAAGAGACAUCACACUCGAtt
CAGUCGUCCUUCACACUAUtt
CCAUAUGAGUUCGAUUUUUtt
- DataSheet:
- FAQs:
Accession NO.: NM_024852
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Product Name
AGO3(NM_024852) Human cDNA/ORF Clone
- Accession NO.
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Transcript Name
Homo sapiens argonaute 3, RISC catalytic component (AGO3), transcript variant 1, mRNA.
- Synonyms
-
ORF length
2583
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Annotation
This variant (1) represents the longer transcript, and encodes the longer isoform (a).
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siRNA
GAAGAGACAUCACACUCGAtt
CAGUCGUCCUUCACACUAUtt
CCAUAUGAGUUCGAUUUUUtt
- DataSheet:
- FAQs:
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Vector Name
pEXP-Entry
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primers
T7: TAATACGACTCACTATAGG
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
4919
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Kanamycin
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Selection Marker
Neomycin
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Protein Tag
Myc,FLAG
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Description
ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-FLAG tags.
Such clones are the best for detection and purification of the transgene using anti-Myc or anti-FLAG antibodies.
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Vector Name
pUC18c
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primers
M13 fwd: GTAAAACGACGGCCAGT
M13 rev: CAGGAAACAGCTATGAC -
Vector Length
2693
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Vector Type
Cloning Vector
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Promoter
lac
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Antibiotic Resistance
Ampicillin
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Selection Marker
-
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Protein Tag
None
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Description
pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for this Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
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Vector Name
pcDNA3.1-3xFlag
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primers
T7: TAATACGACTCACTATAGGG
BGH: TAGAAGGCACAGTCGAGG -
Vector Length
5508
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Vector Type
Mammalian Expression Vector
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Promoter
CMV
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Antibiotic Resistance
Ampicillin
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Selection Marker
Neomycin
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Protein Tag
Flag
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Description
Mammalian vector for expressing C-terminal 3xFlag tagged proteins.
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