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  • Anti-Tie2(CD202b)/TEK antibody
  • Anti-Tie2(CD202b)/TEK antibody

Anti-Tie2(CD202b)/TEK antibody

Cat.#: 105620

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Product Information

  • Product Name
    Anti-Tie2(CD202b)/TEK antibody
  • Documents
  • Description
    Mouse monoclonal to Tie2(CD202b)/TEK
  • Tested applications
  • Species reactivity
    Human Tie2 / CD202b / TEK
    No cross-reactivity in ELISA with Human Tie1; Human Ang2
  • Alternative names
    AA517024 antibody; Cd202b antibody; CD202B antibody; Hyk antibody; RP23-345A23.1 antibody; Tek antibody; TEK antibody; Tie2 antibody; TIE2 antibody; tie-2 antibody; TIE-2 antibody; VMCM antibody; VMCM1 antibody; TIE2 antibody; VMCM antibody; TIE-2 antibody; VMCM1 antibody; CD202B antibody; Hyk antibody; STK1 antibody; Tie2 antibody; Tie-2 antibody; Cd202b antibody; AA517024 antibody
  • Immunogen
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tie2 / CD202b / TEK (rh Tie2 / CD202b / TEK; NP_000450.2; Met1-Lys745). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    FCM: 0.5-2 μg/Test

    ICC/IF: 10-25 μg/mL

  • Validations

    Tie2 / CD202b / TEK Antibody, Mouse MAb, Immunofluorescence

    Tie2 / CD202b / TEK Antibody, Mouse MAb, Immunofluorescence

    Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Tie2 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.

    Tie2 / CD202b / TEK Antibody, Mouse MAb, Flow cytometric analysis

    Tie2 / CD202b / TEK Antibody, Mouse MAb, Flow cytometric analysis

    Flow cytometric analysis of Human TIE2(CD202b) expression on HUVEC cells. Cells were stained with purified anti-Human TIE2(CD202b), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  • Background
    TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
  • References
    • Jones N, et al. (1998) The Tek / Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108.
    • Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27.
    • Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK / TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.