Anti-SYNE1 antibody

Cat.#: 176575

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Product Information

  • Product Name
    Anti-SYNE1 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to SYNE1
  • Tested applications
    WB, ICC, IF, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    8B antibody; AMC3 antibody; AMCM antibody; CPG2 antibody; ARCA1 antibody; EDMD4 antibody; KASH1 antibody; MYNE1 antibody; Nesp1 antibody; SCAR8 antibody; C6orf98 antibody; dJ45H2.2 antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    ICC: 1:200-1:1,000

    IHC-P: 1:50-1:200

    FC: 1:50-1:100

  • Validations

    Fig1: Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.; Positive control:; Lane 1: A549; Lane 2: Mouse spleen tissue

    Fig1: Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution.; Positive control:; Lane 1: A549; Lane 2: Mouse spleen tissue

    Fig2: ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Nesprin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining Nesprin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.

    Fig10: Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the seco

    Fig10: Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the seco

  • Background
  • References
    • Zhang Q et al. Nesprins: a novel family of spectrin-repeat-containing proteins that localize to the nuclear membrane in multiple tissues. J Cell Sci 114:4485-4498 (2001).
    • Stewart-Hutchinson P J et al. Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness. Exp Cell Res 314: 1892-1905 (2008).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"