Rabbit polyclonal Anti-succinyllysine Antibody
ELISA, WB, IF, IP
Anti-succinyllysine antibody detects proteins post-translationally modified by lysine succinylation. The antibody recognizes succinyl lysine in a wide range of sequence contexts. The antibody has been shown to have more than 500 times selectivity against acetylated BSA and unmodified BSA.
The rabbit-derived pan lysine succinylated antibody is purified by succinyl-lysine agarose affinity chromatography. It specifically recognizes proteins with lysine succinylated residues but not the acetyllysine or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation and immunofluorescence, the antibody has been well utilized for proteomic screening to lysine succinylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.
Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.
Store product at -20 ℃. Avoid repeated freeze / thaw. Stable for 12 months from date of receipt.
Recommended antibody dilution: WB: 1:1000; IF: 1:500; IP: 1:200
Important:For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4 degree centigrade; with gentle shaking, overnight.
Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.
Lysine succinylation is a recently identified novel Protein Post-translational Modification. Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both eukaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"