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  • Anti-ROBO4 antibody

Anti-ROBO4 antibody

Cat.#: 104361

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Product Information

  • Product Name
    Anti-ROBO4 antibody
  • Documents
  • Description
    Rabbit monoclonal to ROBO4
  • Tested applications
    ELISA
  • Species reactivity
    Human ROBO4
  • Alternative names
    ECSM4 antibody; FLJ20798 antibody; UNQ421/PRO3674 antibody; 1200012D01Rik antibody; AI593217 antibody; MGC133352 antibody; MGC133353 antibody; MRB antibody; Robo4 antibody; ROBO4 antibody; MRB antibody; ECSM4 antibody; AI593217 antibody; 1200012D01Rik antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human ROBO4 (rh ROBO4; Q8WZ75-1; Met1-Glu469).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ROBO4. The detection limit for Human ROBO4 is approximately 0.0049 ng/well.

  • Validations
  • Background
    Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.
  • References
    • Huminiecki L., et al., 2002, Genomics 79:547-552.
    • Park,K.W. et al., 2003,Dev Biol. 261 (1):251-67.
    • Yoshikawa,M. et al., 2008, Protein Expr Purif. 61 (1):78-82.
    • Jones,C.A. et al., 2008, Nat Med. 14 (4):448-53.
    • Koch,A.W. et al., 2011, Dev Cell. 20 (1):33-46.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"