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  • Anti-PPM1A/PP2CA antibody

Anti-PPM1A/PP2CA antibody

Cat.#: 102934

Special Price 145.0 USD

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Product Information

  • Product Name
    Anti-PPM1A/PP2CA antibody
  • Documents
  • Description
    Rabbit polyclonal to PPM1A/PP2CA
  • Tested applications
  • Species reactivity
    Mouse PPM1A / PP2C-alpha
  • Alternative names
    2310003C21Rik antibody; 2900017D14Rik antibody; AI427932 antibody; AU017636 antibody; FLJ42306 antibody; MGC9201 antibody; MMPa-2 antibody; MPPa-1 antibody; PP2CA antibody; PP2CA antibody; PP2Calpha antibody; PP2C-alpha antibody; PP2C-ALPHA antibody; Ppm1a antibody; PP2CA antibody; PP2Calpha antibody; PP2C-ALPHA antibody; MMPa-2 antibody; MPPa-1 antibody; PPM1A antibody; AI427932 antibody; AU017636 antibody; 2310003C21Rik antibody; 2900017D14Rik antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Mouse PPM1A (rM PPM1A; NP_032936.1; Met 1-Trp 382). Total IgG was purified by Protein A affinity chromatography
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Mouse PPM1A. The detection limit for Mouse PPM1A is 0.0049 ng/well.

  • Validations
  • Background
    Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.
  • References
    • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
    • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
    • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.