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  • Anti-PCNA antibody
  • Anti-PCNA antibody
  • Anti-PCNA antibody
  • Anti-PCNA antibody
  • Anti-PCNA antibody

Anti-PCNA antibody

Cat.#: 141627

Special Price 145.0 USD

Availability: In Stock
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Product Information

  • Product Name
    Anti-PCNA antibody
  • Documents
  • Description
    Rabbit Polyclonal to Human PCNA
  • Tested applications
    WB, IHC-P, ICC/IF, IF, IP
  • Species reactivity
    Human PCNA
  • Alternative names
    MGC8367 antibody; RP23-33N15.1 antibody; ATLD2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PCNA, and purified by antigen affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 1-5 μg/ml

    IHC-P: 0.1-2 μg/mL

    ICC/IF: 0.5-3 μg/mL

    IP: 1-4 μl/mg of lysate

  • Validations

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation

    PCNA Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation

  • Background
    Proliferating Cell Nuclear Antigen (PCNA) is a protein only expresse in nomal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"