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  • Anti-NGF / NGFB antibody

Anti-NGF / NGFB antibody

Cat.#: 100201

Special Price 290.3 USD

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Product Information

  • Product Name
    Anti-NGF / NGFB antibody
  • Documents
  • Description
    Rabbit monoclonal to NGF / NGFB
  • Tested applications
  • Species reactivity
    Human NGF / NGFB
    Has cross-reactivity with Mouse β-NGF (50385-MNAC) in ELISA assay
  • Alternative names
    NGFB antibody; HSAN5 antibody; Beta-NGF antibody; Ngfb antibody; Beta-NGF antibody; HSAN5 antibody; MGC161426 antibody; MGC161428 antibody; NGF antibody; NGFB antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human NGF / NGFB (rHuman NGF / NGFB; NP_002497.2; Ser122-Arg239) and was produced using recombinant antibody technology.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications
    Block - This β-NGF Neutralizing Antibody can block the binding of recombinant β-NGF to its recombinant receptor TrkA.
    Neutralization - The neutralization activity of β-NGF Neutralizing Antibody is Measured by its ability to neutralize β-NGF induced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 17-70 ng/mL in the presence of 220 ng/mL Recombinant Human β-NGF.
  • Validations

    NGF / NGFB Neutralizing Antibody

    NGF / NGFB Neutralizing Antibody

    Cell Proliferation Induced by β-NGF was Neutralized by Human β-NGF Antibody. Recombinant Human β-NGF (Catalog 11050-HNAC) stimulates proliferation in the TF-1 human erythroleukemic cell line. Proliferation elicited by 220 ng/mL of Recombinant Human β-NGF is neutralized by increasing concentrations of Rabbit Anti-Human β-NGF neutralizing Monoclonal Antibody (Catalog 11050-RC6). The IC50 is typically 17-70 ng/mL.

  • Background
    Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
  • References
    • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
    • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
    • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.