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  • Anti-LAMP-1/CD107a/LAMP1 antibody
  • Anti-LAMP-1/CD107a/LAMP1 antibody
  • Anti-LAMP-1/CD107a/LAMP1 antibody
  • Anti-LAMP-1/CD107a/LAMP1 antibody
  • Anti-LAMP-1/CD107a/LAMP1 antibody
  • Anti-LAMP-1/CD107a/LAMP1 antibody

Anti-LAMP-1/CD107a/LAMP1 antibody

Cat.#: 102542

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Product Information

  • Product Name
    Anti-LAMP-1/CD107a/LAMP1 antibody
  • Documents
  • Description
    Rabbit monoclonal to LAMP-1/CD107a/LAMP1
  • Tested applications
    ELISA, WB, IHC-P, FCM, IF, IP, ICC/IF
  • Species reactivity
    Human LAMP1 / CD107a
  • Alternative names
    CD107a antibody; Lamp-1 antibody; AI196048 antibody; LAMPA antibody; CD107a antibody; LGP120 antibody; AI196048 antibody; CD107a antibody; CD107a antibody; LAMP1 antibody; Lamp1 antibody; LAMP-1 antibody; LAMPA antibody; LGP120 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human LAMP1 extracellular domain (rh LAMP1; NP_005552.3; Met 1-Met 382).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 5-10 μg/mL

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LAMP1. The detection limit for Human LAMP1 is 0.00245 ng/well.

    IHC-P: 1-10 μg/mL

    FCM: 0.5-2 μg/Test

    ICC/IF: 10-25 μg/mL

    IP: 0.5-1 μg/mg of lysate

  • Validations

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunofluorescence

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunofluorescence

    Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LAMP1 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunohistochemistry

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunohistochemistry

    Immunochemical staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunohistochemistry

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunohistochemistry

    Immunochemical staining of human LAMP1 in human placenta with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunoprecipitation

    LAMP1 / CD107a Antibody, Rabbit MAb, Immunoprecipitation

    LAMP1 / CD107a Antibody, Rabbit MAb, Western blot

    LAMP1 / CD107a Antibody, Rabbit MAb, Western blot

    LAMP-1 / CD107a / LAMP1 Antibody, Rabbit MAb, Flow cytometric analysis

    LAMP-1 / CD107a / LAMP1 Antibody, Rabbit MAb, Flow cytometric analysis

    Flow cytometric analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  • Background
    Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I membrane protein which belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells, but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in whom amyloid burden was cleared following betaA-peptide immunization. In addition, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.
  • References
    • Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.
    • Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.
    • Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"