NovoPro
Cart 2
  • Anti-IFN gamma antibody

Anti-IFN gamma antibody

Cat.#: 105427

Special Price 161.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-IFN gamma antibody
  • Documents
  • Description
    Rabbit monoclonal to IFN gamma
  • Tested applications
    FCM
  • Species reactivity
    Human IFN gamma / IFNG
  • Alternative names
    gamma interferon antibody; Ifg antibody; IFG antibody; IFI antibody; IFN gamma antibody; IFN gamma antibody; IFNG antibody; Ifng antibody; IFN-g antibody; immune interferon antibody; interferon gamma antibody; Ifg antibody; IFN-g antibody; IFG antibody; IFI antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human IFN gamma / IFNG (rh IFN gamma / IFNG; NP_000610.2; Met1-Gln166) and conjugated with PE under optimum conditions, the unreacted PE was removed.
  • Clonality
    Monoclonal
  • Formulation
    Aqueous solution containing 0.5% BSA and 0.09% sodium azide
  • Storage instructions
    This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
    Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
  • Applications
    FCM
  • Validations

    IFN gamma / IFNG Antibody (PE), Rabbit MAb, Flow Cytometry

    IFN gamma / IFNG Antibody (PE), Rabbit MAb, Flow Cytometry

    Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Human peripheral blood mononulcear cells were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with PE-conjugated anti-Human IFN-γ and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

  • Background
    IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
  • References
    • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
    • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
    • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
    • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
    • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
    • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"