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Product Name
Anti-GGCT antibody
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Description
Rabbit polyclonal to GGCT
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human GGCT
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Alternative names
GGC antibody; GCTG antibody; CRF21 antibody; C7orf24 antibody; Ggc antibody; Gctg antibody; AA673177 antibody; A030007L17Rik antibody; A030007L17Rik antibody; AA673177 antibody; C7orf24 antibody; CRF21 antibody; cytochrome c-releasing factor 21 antibody; gamma -glutamyl cyclotransferase antibody; gamma-glutamyl cyclotransferase antibody; gamma-glutamylcyclotransferase antibody; gamma-glutamylcyclotransferase antibody; Gctg antibody; GCTG antibody; Ggc antibody; GGC antibody; Ggct antibody; GGCT antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human GGCT (rh GGCT; O75223-1; Met1-Leu188). GGCT specific IgG was purified by Human GGCT affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GGCT. The detection limit for Human GGCT is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 1-4 μg/mg of lysate
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Background
GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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References
- Wagner SA. et al., 2011, Mol Cell Proteomics. 10 (10): M111.013284.
- Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
- Amano T. et al., 2012, J Histochem Cytochem. 60 (1): 76-86.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"