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  • Anti-EGFR/HER1/ErbB1 antibody

Anti-EGFR/HER1/ErbB1 antibody

Cat.#: 100592

Special Price 290.3 USD

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Product Information

  • Product Name
    Anti-EGFR/HER1/ErbB1 antibody
  • Documents
  • Description
    Rabbit monoclonal to EGFR/HER1/ErbB1
  • Tested applications
  • Species reactivity
    Human EGFR / HER1 / ErbB1
    Has cross-reactivity with Rhesus EGFR (90317-K08H) in ELISA assay
    No cross-reactivity with Mouse EGFR (51091-M08H) and Rat EGFR (80100-R08H) in ELISA assay
  • Alternative names
    ERBB antibody; HER1 antibody; mENA antibody; ERBB1 antibody; PIG61 antibody; NISBD2 antibody; Wa5 antibody; wa2 antibody; Erbb antibody; Errp antibody; wa-2 antibody; Errb1 antibody; AI552599 antibody; 9030024J15Rik antibody; 9030024J15Rik antibody; AI552599 antibody; EGFR antibody; Egfr antibody; Egfr antibody; ERBB antibody; Erbb antibody; ERBB1 antibody; ERBB1 antibody; ErbB-1 antibody; Errp antibody; Errp antibody; HER1 antibody; mENA antibody; PIG61 antibody; RP23-295E4.1 antibody; wa2 antibody; wa-2 antibody; Wa5 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; NP_005219; Met1-Ser645) and was produced using recombinant antibody technology.
  • Clonality
  • Formulation
    0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications
    Block - This EGFR Neutralizing Antibody can block the binding of recombinant EGF to its recombinant receptor EGFR.
    Neutralization - The neutralization activity of EGFR Neutralizing Antibody is Measured by its ability to neutralize autocrine EGF induced proliferation in the human breast cancer MDA-MB-468 cell line. The Neutralization titer (IC50) is typically 0.05-0.2 μg/mL for 5000 cells/well.
  • Validations

    EGFR / HER1 / ErbB1 Neutralizing Antibody

    EGFR / HER1 / ErbB1 Neutralizing Antibody

    Cell Proliferation Induced by Autocrine EGF was Neutralized by Human EGFR Antibody. Autocrine EGF stimulates proliferation in the human breast cancer MDA-MB-468 cell line. Proliferation elicited by Autocrine EGF is neutralized by increasing concentrations of Human EGFR Monoclonal Antibody (Catalog 10001-RE01). The IC50 is typically 0.05-0.2 μg/mL.

  • Background
    As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
  • References
    • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
    • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
    • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.