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Product Name
Anti-DYKDDDDK-tag Mouse mAb(HRP-Conjugate)
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Description
DYKDDDDK-tag Mouse mAb(HRP-Conjugate)
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Tested applications
WB, IP
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Species reactivity
Transfected
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Alternative names
FLAG antibody
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Isotype
IgG2b
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Preparation
Antigen: Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
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Clonality
Monoclonal
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Formulation
In PBS(pH 7.4) containing with 1mg/ml BSA 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different FLAG-fusion proteins (B,C,D), using DYKDDDDK-tag-HRP (HRP-Conjugate) Mouse mAb (1:1000 diluted).
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Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"