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Product Name
Anti-DKK-1 antibody
- Documents
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Description
Rabbit monoclonal to DKK-1
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Tested applications
ELISA, WB
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Species reactivity
Human Dkk1 / Dickkopf-1
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Alternative names
S antibody; SK antibody; DKK-1 antibody; mdkk-1 antibody; Dickkopf 1 antibody; DKK1 antibody; DKK-1 antibody; hDKK 1 antibody; SK antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human dickkopf related protein 1 (rh Dkk1; Met 2-His 266; NP_036374.1).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Dkk1. The detection limit for Human Dkk1 is approximately 0.00975 ng/well.
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Validations
Dkk1 / Dickkopf-1 Antibody, Rabbit MAb, Western blot
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Background
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
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References
- Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.
- Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"