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  • Anti-crotonyllysine mouse monoclonal antibody

Anti-crotonyllysine mouse monoclonal antibody

Cat.#: 106771

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Product Information

  • Product Name
    Anti-crotonyllysine mouse monoclonal antibody
  • Documents
  • Description
    Mouse monoclonal Anti-crotonyllysine antibody
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Anti-crotonyllysine monoclonal antibody detects proteins post-translationally modified by lysine crotonylation. The antibody recognizes crotonyllysine in a wide range of sequence contexts, both in histones and non-histone substrates.
  • Isotype
    Mouse IgG
  • Preparation
    The pan anti-crotonyllysine monoclonal antibody is developed by using crotonylated-KLH conjugates as immunogen. The product specifically recognizes proteins with lysine crotonylated residues but not the structurally-similar modified lysine residues or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry, the antibody is with ChIP grade quality and has been well utilized for proteomic screening to lysine crotonylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.
  • Clonality
    Monoclonal
  • Formulation
    Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.
  • Storage instructions
    Store product at -20 ℃. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt.
  • Applications

    For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4 degree centigrade; with gentle shaking, overnight.

    Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

  • Validations

    A. Dot blotting analysis on 10 ng of lysine crotonylated peptide library (A1), lysine crotonylated BSA (B1), selected histone lysine crotonylated peptides (A2-7, B2-6, C1-6), unmodified lysine peptide library (B7), unmodified BSA (C7), lysine acetylated peptide library (A8), lysine propionylated peptide library (B8), lysine butyrylated peptide library (C8) and randomly selected histone unmodified peptides (A9, B9, C9) using anti-crotonyllysine mAb. B. Western blotting analysis on 30 ug of HeLa crude proteins using anti-crotonyllysine mAb.

    A. Dot blotting analysis on 10 ng of lysine crotonylated peptide library (A1), lysine crotonylated BSA (B1), selected histone lysine crotonylated peptides (A2-7, B2-6, C1-6), unmodified lysine peptide library (B7), unmodified BSA (C7), lysine acetylated peptide library (A8), lysine propionylated peptide library (B8), lysine butyrylated peptide library (C8) and randomly selected histone unmodified peptides (A9, B9, C9) using anti-crotonyllysine mAb. B. Western blotting analysis on 30 ug of HeLa crude proteins using anti-crotonyllysine mAb.

  • Background
    Lysine crotonylation is a newly-identified histone post-translational modification by integrated proteomic approaches and elaborate biochemistry analyses. It has been shown that lysine crotonylation is an evolutionarily conserved PTM in multiple species of both prokaryotes and eukaryotes in a wide range of proteins, both in histones and non-histone substrates. The unique structure and genomic localization of lysine crotonylation suggest that it is mechanistically and functionally different from lysine acetylation. Given the revealed roles of lysine crotonylation in spermatogenesis, the novel modification may play important roles in multiple cellular pathophysiological processes by effecting chromatin structure and epigenetic pathways.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"