Rabbit Polyclonal to Human CDK1
WB, ICC/IF, IF, IP
Human CDC2 Kinase / CDK1
CDC2 antibody; Cdc2 antibody; CDC2> antibody; CDC28A antibody; Cdc2a antibody; CDK1 antibody; Cdk1 antibody; DKFZp686L20222 antibody; MGC111195 antibody; p34< antibody; P34CDC2 antibody; CDC2 antibody; CDC28A antibody; P34CDC2 antibody; Cdc2 antibody; Cdc2a antibody; p34< antibody; CDC2> antibody
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CDC2 Kinase / CDK1, and purified by antigen affinity chromatography.
0.2 μm filtered solution in PBS
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
WB: 1-5 μg/ml
IP: 1-4 uL/mg of lysate
Human CDC2 Kinase / CDK1 Western blot (WB) 15736
Human CDC2 Kinase / CDK1 Immunofluorescence(IF) 15737
Human CDC2 Kinase / CDK1 Immunoprecipitation(IP) 15738
CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"