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  • Anti-CD112/Nectin-2/PVRL2 antibody

Anti-CD112/Nectin-2/PVRL2 antibody

Cat.#: 101738

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Product Information

  • Product Name
    Anti-CD112/Nectin-2/PVRL2 antibody
  • Documents
  • Description
    Mouse monoclonal to CD112/Nectin-2/PVRL2
  • Tested applications
  • Species reactivity
    Human CD112 / Nectin2
    No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
  • Alternative names
    nectin-2 antibody; nectin-2 antibody; PRR2 antibody; PRR2 antibody; Pvrl2 antibody; PVRL2 antibody; PVRR2 antibody; HVEB antibody; PRR2 antibody; CD112 antibody; PVRR2 antibody; MPH antibody; Pvr antibody; Cd112 antibody; AI325026 antibody; AI987993 antibody; nectin-2 antibody; PVS antibody; Pvr antibody; PVS antibody; AI325026 antibody; AI987993 antibody; CD112 antibody; CD112 antibody; HVEB antibody; mHVEB antibody; MPH antibody
  • Immunogen
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD112 / Nectin2 extracellular domain (rhCD112; aa1-360; NP_002847.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect CD112. The detection limit for CD112 is approximately 2.5 ng/well.

  • Validations
  • Background
    Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
  • References
    • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
    • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.