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  • Anti-CD10/Neprilysin antibody

Anti-CD10/Neprilysin antibody

Cat.#: 104234

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Product Information

  • Product Name
    Anti-CD10/Neprilysin antibody
  • Documents
  • Description
    Mouse monoclonal to CD10/Neprilysin
  • Tested applications
  • Species reactivity
    Human CD10 / Neprilysin / MME
    No cross-reactivity in ELISA with Human cell lysate (293 cell line)
  • Alternative names
    MME antibody; MME antibody; 6030454K05Rik antibody; C85356 antibody; CALLA antibody; CALLA antibody; CD10 antibody; CD10 antibody; DKFZp686O16152 antibody; MGC126681 antibody; MGC126707 antibody; NEP antibody; NEP antibody; Neprilysin antibody; Neprilysin antibody; Neutral endopeptidase antibody; Neutral endopeptidase antibody; NEP antibody; SFE antibody; CD10 antibody; CALLA antibody; NEP antibody; SFE antibody; CD10 antibody; CALLA antibody; C85356 antibody; 6030454K05Rik antibody
  • Immunogen
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD10 / Neprilysin / MME (rh CD10 / Neprilysin / MME; NP_000893.2; Tyr 52-Trp 750). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human MME. The detection limit for Human MME is approximately 0.16 ng/well.

  • Validations
  • Background
    The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.
  • References
    • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
    • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
    • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
    • Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.