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  • Anti-Carboxypeptidase A2/CPA2 antibody

Anti-Carboxypeptidase A2/CPA2 antibody

Cat.#: 104427

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Product Information

  • Product Name
    Anti-Carboxypeptidase A2/CPA2 antibody
  • Documents
  • Description
    Mouse monoclonal to Carboxypeptidase A2/CPA2
  • Tested applications
    IHC-P
  • Species reactivity
    Human Carboxypeptidase A2 / CPA2
    No cross-reactivity in ELISA with Human CPE; Human CPB1
  • Alternative names
    Carboxypeptidase A2 antibody; CPA2 antibody
  • Immunogen
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carboxypeptidase A2 / CPA2 (rh Carboxypeptidase A2 / CPA2; NP_001859.1; Met 1-Tyr 417). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    IHC-P: 10-30 μg/mL

  • Validations

    Carboxypeptidase A2 / CPA2 Antibody, Mouse MAb, Immunohistochemistry

    Carboxypeptidase A2 / CPA2 Antibody, Mouse MAb, Immunohistochemistry

    Immunochemical staining of human CPA2 in human pancreas (from 2 donors) with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to pancreatic acinus.

  • Background
    Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
  • References
    • Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657.
    • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
    • Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"