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  • Anti-B7-DC/PD-L2/CD273 antibody

Anti-B7-DC/PD-L2/CD273 antibody

Cat.#: 102498


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Product Information

  • Product Name
    Anti-B7-DC/PD-L2/CD273 antibody
  • Documents
  • Description
    Rabbit monoclonal to B7-DC/PD-L2/CD273
  • Tested applications
  • Species reactivity
    Human PD-L2 / CD273 / B7-DC
    Has cross-reactivity in ELISA with Human B7-H2 / ICOSL / CD275; Mouse CD86 / B7-2 / B70; Mouse B7-H1 / CD274 / PD-L1
    No cross-reactivity in ELISA with Human CD80 / B7-1; Human CD86 / B7-2 / B70; Mouse B7-H2 / ICOSL / CD275
  • Alternative names
    B7DC antibody; B7-DC antibody; bA574F11.2 antibody; Btdc antibody; Btdc antibody; CD273 antibody; F730015O22Rik antibody; MGC124039 antibody; MGC124040 antibody; MGC142238 antibody; MGC142240 antibody; PDCD1L2 antibody; PDCD1LG2 antibody; Pdcd1lg2 antibody; PDL2 antibody; PD-L2 antibody; PD-L2 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L2 / CD273 (rh PD-L2; NP_079515.2; Met 1-Pro 219).
  • Clonality
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 2-5 μg/ml

    ELISA: 0.5-1 μg/mL

    This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PDL2. The detection limit for Human PDL2 is 0.00245 ng/well.

  • Validations

    B7-DC / PD-L2 / CD273 Antibody, Rabbit MAb, Western blot

    B7-DC / PD-L2 / CD273 Antibody, Rabbit MAb, Western blot

  • Background
    Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
  • References
    • Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
    • Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
    • Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.