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  • Human Alkaline Phosphatase / ALPL (His Tag) recombinant protein

Human Alkaline Phosphatase / ALPL (His Tag) recombinant protein

Cat.#: 500478

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Product Information

  • Product Name
    Human Alkaline Phosphatase / ALPL (His Tag) recombinant protein
  • Documents
  • Description
    Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
  • Protein name
    Alkaline phosphatase
  • Protein short names
    APTNAP; FLJ40094; AKP2; FLJ93059; MGC167935; TNSALP; ALKALINE PHOSPHATASE; TNAP; AP-TNAP; AKP-2; RP23-277N22.4; HOPS; ALPL; MGC161443
  • Uniprot ID
    P05186
  • Gene Name
    ALPL; hCG_38868
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human tissue-nonspecific alkaline phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
    The specific activity is > 50, 000 pmoles/min/μg.
  • Validations

    Human Alkaline Phosphatase / ALPL Protein (His Tag) SDS-PAGE

    Human Alkaline Phosphatase / ALPL Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"