ADORA2A Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
WB, IHC-P, FC, ELISA
ADORA2 antibody; Adenosine receptor A2a antibody; ADORA2A antibody; ADORA2 antibody
This ADORA2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 347-381 amino acids from the C-terminal region of human ADORA2A.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
FC: 1:25, IHC-P: 1:25, WB: 1:1000
Overlay histogram showing SH-SY5Y cells stained with 137605 (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (137605, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
137605 staining ADORA2A in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Western blot analysis of lysate from SH-SY5Y cell line, using ADORA2A Antibody (C-term)(Cat. #137605). 137605 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
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