Gene Synthesis

Q: What are the applications of gene synthesis?
A: Gene synthesis has a variety of applications, including the creation of specialized cDNA libraries, large-scale production of microarray-ready cDNA, the design of gene therapy vectors, and the synthesis of gene variants.
Q: What is the standard vector for NovoPro gene synthesis?
A: NovoPro offers a variety of vectors for your choice, free of charge, such as pET vectors, pEGFP vectors, lentiviral vectors, siRNA vectors, etc. Customer-provided vectors are also welcome for our expression system.
Q: Can NovoPro subclone the gene into the vector of my choice?
A: NovoPro can subclone the synthetic gene into the vector of your choice for free. The vector (commercial vector or non-commercial vector) must be provided by the customer with a full-length sequence and other requested info.
Q: Are the genes sequencing verified?
A: Yes. The sequences of genes are confirmed by DNA sequencing, and 100% fidelity is guaranteed. For all gene synthesis orders, we provide sequence chromatograms covering your gene (electronic), a construct map for the plasmid (electronic), and a quality assurance certificate.
Q: Why use gene synthesis instead of PCR cloning?
A: PCR cloning, the most common method of obtaining genes, may not be able to produce a gene of sufficient quality, or even at all. First, a cDNA library for a specific type of tissue has to be prepared or purchased, which requires either time or expense. Second, the gene must be abundant in that cDNA library or it will be very difficult to clone in any useful way. Third, when PCR is successful, its products may have mutations or single nucleotide polymorphisms (SNP), which can cause problems during experimentation or other applications. Finally, with PCR cloning you are limited to copying sequences that exist in nature and employing time-consuming, error-prone methods of mutagenesis and recombination to create custom constructs such as reporter genes, fusion proteins, etc; de novo gene synthesis offers complete flexibility to design your sequence, including codon optimization to improve protein expression levels. NovoPro guarantees 100% sequence fidelity and fast delivery of workable quantities of precise, unmutated genes at a far more economical price than PCR cloning.
Q: Is there a length limit for NovoPro gene synthesis?
A: There is no length limit. NovoPro routinely synthesizes genes of 10 kb or longer.
Q: Does codon optimization really matter?
A: It matters as much as protein expression matters. Different organisms have different codon usage preferences. For example, the preferred codons used in a human gene may be rare in bacteria. This can cause problems when researchers attempt to express human genes in E. coli. Using our optimized synthetic genes, many of our customers have reported dramatic increases in protein expression.
Q: How can I place my order?
A: You can give NovoPro your sequence and payment information via a variety of means, such as e-mail, leaving us message at the bottom of the site or online chat.
Q: What is the time frame for delivery?
A: For genes up to 1.5 kb, the delivery time is 10-12 business days. One week is added for each additional 1.5 kb.
Q: How much does it cost to ship?
A: We ship our products to many countries. The shipping charge is determined once a quote is prepared, and depends upon the weight, destination, carrier, and speed of shipment.
Q: What kinds of payments do you accept?
A: NovoPro accepts credit cards (Visa, MasterCard, American Express), checks and wire transfer. You may send us a PO. For certain orders, NovoPro may require a deposit.
Q: What are the steps in the process of gene synthesis?
A: The steps in gene synthesis include designing and synthesizing oligos, assembling oligos to obtain full-length genes, correcting mutations, and confirming the sequence.
Q: What is the delivery package?
A: The delivery specifications for all gene synthesis orders are:
  • Plasmid containing your gene insert
  • Sequence chromatograms covering your gene (electronic)
  • Gene synthesis report (electronic)