Custom Monoclonal Antibody Production

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NovoMonoTM, a Freeland for any desired antibodies

Custom antibody production
 

Leading technologies

NovoFocusTM--focus on the unique sequences, unstructured and exposed regions.

Precise antigens design is vital for successful antibody production. To reach better antibody-antigen recognition of most applications, NovoFocusTM, developed by NovoPro's bioinformatics team, is able to predict, evaluate protein structure information and effectively select the unstructured or loop regions of proteins. To guarantee the specificity, a novel sequence comparison algorithm is developed which can screen the possible peptide sequences in proteome level and calculate similarities in an antibody development view. More than 20 factors have been integrated to the comprehensive NovoFocusTM antigen design, lead to a precise value of epitopes and customer-friendly interface.

The major parameters consist of NovoFocusTM epitope value

Structure Information Protein Regions
α-helix/ β sheet/ loop Signal peptide (avoided)
Amino Acid Characteristics Transmembrane region (avoided)
Antigenic value of amino acid Possibly modified regions
Flexibility Disordered regions
Sequence specificity Solvent accessibility
Blast target species and rabbit N-terminal, C-terminal
Requirements of Customers Evolution
Specified regions Positive selection
Recognize multiple proteins Discrimination
Differentiate specific proteins  

 

NovoBoostTM—best adjuvant leads to highest affinity

Affinity is key for antibody effectiveness, especially important for low abundance proteins and high requirement applications. To greatly improve the antibody, a novel immunogenicity-amplified adjuvant - NovoBoostTM is developed. NovoBoostTM is composed from small (<10kD) dendrimers positively charged at their surface. When the antigen is mixed with NovoBoostTM, the large, positively charged complex molecule is obtained, which effectively binds to antigen receptors on dendritic cells, and effectively stimulate T-helper activity, thus inducing strong and specific immune response. Thanks to the tremendously enhanced immunogenicity, 90% of the NovoPro antibodies could reach 1ng-20ng detection limit (Fig 1.1 & Fig 1.2).

Helpful tech support, experiencing true partnership

Your success is our mission.

NovoPro scientific team has years of antibody validation and application experiences. Not only you can find various of antibody information such as antibody user guide, FAQs, tips, but also you are always welcome to discuss with our experts about any thoughts and problems in experiments. We would try our best to make your research easy and enjoyable.

Product Selection

Monoclonal antibody production using protein as antigen

Protein antigens are the elite choice when it comes to high-quality monoclonal antibody production. Their unique ability to elicit monoclonal antibodies that recognize the native confirmation of the target protein makes them a superior choice over other antigen strategies.


Package NovoMono Protein
Guarantees WB positive for immunogen; ELISA ≥ 1:64,000
Starting Material Target protein name, sequence or accession number NovoPro will produce the protein antigen
Immunization 5 Balb/c mice
Customer Screening 5 ELISA(+) supernatants for evaluation in client's own experimental system
Deliverables 1mg recombinant protein; 2-5 hybridoma cell lines of customer's choice+; 2-5 mg purified mAb from two chosen hybridoma
Final Delivery 21-26 weeks
Cost $2,998

*additional cell lines and purified antibody can be provided as $399/clone and $399/2mg, clone respectively


Monoclonal antibody production using peptide as antigen

In cases where access to a target protein is limited or unavailable, but the amino acid sequence is known, peptide antigens offer a smart solution for monoclonal antibody production. Peptide antigens are also ideal for producing monoclonal antibodies against post-translational modification sites within a target protein.


Package Starting Material Antigen Immunization Customer Screening First Delivery Cost
NovoMonoTM Protein Step 1 Target protein name, sequence or accession number. NovoPro will design and produce the 4 peptide antigens 4 Balb/c mice 4 ELISA(+) sera in client's own experimental system 10-12 weeks $999

The customer chooses the best 2 animals from among the original 5 mice. These best-responding animals are boosted twice more and then their spleen cells harvested for hybridoma fusion (10 plates per fusion). An ELISA assay is used to screen all positive supernatants, and in the end 2-5 best cell lines will be chosen and proceed to subcloning and final hybridoma.


Package Starting Deliverables Second Delivery Cost
NovoMonoTM Protein Step 2 2 Chosen mice 2-5 hybridoma clones*
Pre-immune sera as controls
2-5mg purified antibodies from two cell lines.
2-3mg Purified peptides
Project report
10-12 weeks $1998

*additional cell lines and purified antibody can be provided as $399/clone and $399/2mg, clone respectively


Timelines

STEP Estimated Time Description

STEP1:

Preimmune

Day 0

 

1st Immunization

Day 1

 

2nd Immunization

Day 14

 

3rd Immunization

Day 21

 

Test Bleed & ELISA Test

Day 28

 

4th Immunization

Day 39

 

5th Immunization

Day 60

Optional

6th Immunization

Day 68

Optional

STEP2 & STEP3:

Spleen cell Isolation & Fusion

Day 43 or Day 70

Depending on the titer we can perform additional immunizations before fusion

STEP4:

ELISA test and QC

Day 53 or Day 80

STEP5:

Subcloning, Production and purification

Day 83 or Day 110

 

Most thoughtful service procedure

Project inquiry

Tel: +86-021-61842887
Email: support@novoprolabs.com
Web: www.novoprolabs.com/

Project initial plan

Contact our experienced antibody consultant and form a brief antibody development plan together. 

Service agreement

Customer confirmation of the proposal, a service agreement signed by both parties. If possible, a Purchase Order (PO) is desired to be issued by the financial office.

Project initiation

Project(s) will be initiated immediately on the receive of the agreement or PO.

Project status

Customer will receive an account in Customer Center to track the project status, the status will be updated once a week.

Sending QC reports

The antibody QC reports will be sent to customer when the project completes, and a payment or PO is expected to be made by the successful completion of the project.

Delivery

Delivery through DHL, domestic: 2-3 working days, oversea: 3-5 working days.

Tech support

Any question about antibody validation or suggestions, please contact: support@novoprolabs.com