Basic Vector Information
- Vector Name:
- 4xCSL-luciferase
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5715 bp
- Type:
- Mammalian Expression, Luciferase
- Replication origin:
- ori
- Copy Number:
- High Copy
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- F1ori-F (GTGGACTCTTGTTCCAAACTGG)
- 3' Primer:
- LucNRev
4xCSL-luciferase vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
4xCSL-luciferase vector Sequence
LOCUS 40924_90 5715 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5715) AUTHORS Saxena MT, Schroeter EH, Mumm JS, Kopan R TITLE Murine notch homologs (N1-4) undergo presenilin-dependent proteolysis. JOURNAL J Biol Chem. 2001 Oct 26;276(43):40268-73. Epub 2001 Aug 22. PUBMED 11518718 REFERENCE 2 (bases 1 to 5715) TITLE Direct Submission REFERENCE 3 (bases 1 to 5715) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Chem."; date: "2001-10-26"; volume: "276(43)"; pages: "40268-73. Epub 2001 Aug 22" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5715 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal 86..207 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS 425..2074 /codon_start=1 /label=luciferase /note="firefly luciferase" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL" intron 2318..2383 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 2513..2533 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 2958..3092 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3234..3251) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3405..3993) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4167..5024) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5025..5129) /label=AmpR promoter rep_origin 5156..5611 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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